Part:BBa_K4165172
T7 promoter- His tag- WAP-T7 terminator
This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), WAP Inhibitor (BBa_K4165008), and T7 terminator (BBa_K731721), The His tag was attached to the WAP inhibitor coding sequence to serve in the purification using NI-NTA column.
Usage and Biology
Human serine protease inhibitor WAP-four disulfide core domain 13 is able to inhibit HtrA1 (BBa_K4165004) through binding with Htra1 through targeting and degrading both tau and Aβ proteins which are both considered the main causes of Alzheimer’s Disease pathogenesis
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 379
Illegal AgeI site found at 115 - 1000COMPATIBLE WITH RFC[1000]
Dry Lab
Mathematical modeling
Transcription rate and translation rate under T7 promotor
the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab.
Figure 1. this figure shows the results from the transcription and translation code showing the
variation of mRNA and protein concentrations with time compared with the wet lab results.
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